Fig. 4

MRCCAT1 overexpression promotes ccRCC cells proliferation, migration and invasion in vitro. a Relative expression of MRCCAT1 in pcDNA3.1⁺-MRCCAT1 transfected compared with pcDNA3.1⁺-vector transfected 786-O and Caki-1 cells. b CCK-8 assay of MRCCAT1 overexpressed and NC ccRCC cells at indicated times. c, d Upper: representative images of the wound-healing assay of MRCCAT1 overexpressed compared with negative control 786-O (c) and Caki-1 cells (d), photographed at indicated times after scratching. Scale bar = 800 μm. Lower: the relative breath after migration was measured and calculated among the groups. e, f Left: Transwell assays were performed to evaluate the invasion of MRCCAT1 overexpressed compared with NC 786-O (e) and Caki-1 cells (f). Scale bar = 200 μm. Right: The statistical graph indicates the number of cells averaged from 8 random high power fields. The results are presented as the mean ± SD from three independent experiments; *P < 0.05, **P < 0.01, ***P < 0.001 by Student’s t-test