Skip to main content
Fig. 3 | Molecular Cancer

Fig. 3

From: PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway

Fig. 3

Effect of PRC1 gene silencing on the growth of lung adenocarcinoma in vitro. a PRC1 expression was detected by western blot analysis and qPCR in NSCLC cell lines (A549, SPC-A1, H1299, H1703, and SK-MES-1). β-actin was used as a loading control. b PRC1 expression was confirmed by qPCR and western blot analysis in lung adenocarcinoma cells (A549, SPC-A1, and H1299) transduced with lentiviruses expressing scramble (shControl) or PRC1-targeting (shPRC1–1 and shPRC1–2) shRNA. Cells were harvested 5 days after viral transduction. β-actin was used as a loading control. c MTT assay was performed to determine the proliferation of lentiviruses shPRC1 transfected A549, SPC-A1, and H1299 cells. d A colony formation assay of A549, SPC-A1, and H1299 cells transduced with lentiviruses expressing the indicated shRNA. Cells were grown for 10–14 days under the selection of puromycin (0.25 mg/ mL) and stained with crystal violet. The colonies were counted and captured. The data represent the mean ± S.D. from three independent experiments. **P < 0.01, ***P < 0.005

Back to article page