Fig. 5

Overexpression of PRC1 promotes on the proliferation in A549 and HBE cells a qRT-PCR and Western blotting analysis of PRC1 mRNA and protein level in A549 cells transfected with PRC1 expressing plasmid or empty vector. β-Actin mRNA expression was used as an internal control. Experiment was conducted in triplicate. *** P < 0.005. b A colony formation assay of A549 cells transduced with PRC1 expressing plasmid or empty vector. The colonies were counted and captured. The data represent the mean ± S.D. from three independent experiments, *P < 0.05. c PRC1 mRNA and protein level in HBE cells transfected with PRC1 expressing plasmid or empty vector, *** P < 0.005. d A colony formation assay of HBE cells transduced with PRC1 expressing plasmid or empty vector, *P < 0.05