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Fig. 8 | Molecular Cancer

Fig. 8

From: PRC1 contributes to tumorigenesis of lung adenocarcinoma in association with the Wnt/β-catenin signaling pathway

Fig. 8

PRC1 correlated the WNT pathway through NGS analysis in vitro. a RNA transcriptome sequencing analysis was performed to analyze the gene expression profile of A549 cells transduced with shPRC1–1 or shControl. A volcano plot showed the differentially expressed genes. b KEGG pathway analysis for all genes with altered expression between the negative control group and shPRC1–1 knockdown group cells in vitro. c Expression heatmap of Wnt pathway genes significantly differentially expressed transcripts after the transduction of shPRC1–1. Expression profiles of the 44 probes in A549 cells with shControl (left) and shPRC1–1 (right) are shown. Red and green indicate up- and downregulation, respectively. d qRT-PCR analysis of the mRNA expression levels of 16 Wnt pathway relevant DEGs in shControl vs. shPRC1–1-treated A549 cells and SPC-A1. e Western blot analysis of the level of 16 Wnt pathway relevant DEGs in shControl vs. shPRC1–1-treated A549 cells and SPC-A1

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Molecular Cancer

ISSN: 1476-4598