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Fig. 3 | Molecular Cancer

Fig. 3

From: Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p

Fig. 3

Linc00673 was required for epithelial mesenchymal transition. a. Expression of Vimentin, N-cadherin, Snail, ZEB1 and E-cadherin in TGF-β treated A549 cells as determined by western blot. b. Expression of Vimentin and E-cadherin in TGF-β receptor antagonist SB431542 and TGF-β treated A549 cells as determined by western blot. c. Expression of linc00673 in TGF-β and SB431542 treated A549 cells as determined by qRT-PCR. d. Expression of linc00673 in TGF-β and SB431542 treated H1975 cells as determined by qRT-PCR. e. Expression of EMT markers in si-NC or si-L3/si-L5 transfected followed by TGF-β treated A549 and H1975 cells as determined by western blot. f. mRNA level of EMT markers in si-NC or si-L3 transfected followed by TGF-β treated A549 and H1975 cells as determined by qRT-PCR. g. Immunofluorescence staining of Vimentin expression in si-NC or si-L3 transfected followed by TGF-β treated A549 and H1975 cells. Error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005, #p < 0.05, ##p < 0.01, ###p < 0.005

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