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Fig. 5 | Molecular Cancer

Fig. 5

From: Long non-coding RNA linc00673 regulated non-small cell lung cancer proliferation, migration, invasion and epithelial mesenchymal transition by sponging miR-150-5p

Fig. 5

Linc00673 acted as a ceRNA by sponging miR-150-5p and regulated ZEB1 expression indirectly. a. 293 T cells were co-transfected with miR-150-5p mimics or mimics NC and pmirGLO-ZEB1–3’UTR-WT or pmirGLO-ZEB1–3’UTR-MUT. Luciferase activity was detected 24 h after transfection using the dual luciferase assay. b. A549 cells were co-transfected with miR-150-5p mimics or mimics NC and pmirGLO-ZEB1–3’UTR-WT or pmirGLO-ZEB1–3’UTR-MUT. Luciferase activity was detected 24 h after transfection using the dual luciferase assay. c. Predictive binding sites of miR-150-5p to linc00673 and schematic of wild-type and mutant pmirGLO-linc00673 constructs. d. 293 T cells were co-transfected with miR-150-5p mimics or mimics NC and pmirGLO-linc00673-WT or pmirGLO-linc00673-MUT. Luciferase activity was detected 24 h after transfection using the dual luciferase assay. e. A549 and H1975 cells were co-transfected with miR-150-5p mimics or mimics NC and pmirGLO-linc00673-WT or pmirGLO-linc00673-MUT. Luciferase activity was detected 24 h after transfection using the dual luciferase assay. Error bars indicate the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.005

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Molecular Cancer

ISSN: 1476-4598