Fig. 4

RNA microarray and pathway analyses revealed that RAB3C regulates the IL-6 signaling pathway (a) Putative probes regulated by RAB3C were identified from genes upregulated/downregulated at least 2-fold in CX-1 RAB3C cells compared with vector control cells. P < 0.05 was considered significant enrichment. b An RNA microarray analysis showed that RAB3C overexpression induced the expression of many cytokines. The IL-6 pathway was the top pathway hose members exhibited gene expression changes after RAB3C overexpression, according to Ingenuity Pathway Analysis (IPA). c IPA upstream pathway analysis of mass spectrometry proteomics data also revealed that IL-6 is a key downstream pathway in RAB3C-overexpressing cells. d Correlation between the endogenous RAB3C protein level and IL-6 activity in colon cancer cell lines