Fig. 1

miR-210-3p is upregulated in bone metastatic PCa tissues and cells. a miR-210-3p expression levels was markedly elevated in PCa tissues compared with that in adjacent normal tissues (ANT) as assessed by analyzing the TCGA PCa miRNA sequencing dataset (ANT, n = 52; PCa, n = 498). *P < 0.05. b Real-time PCR analysis of miR-210-3p in 52 paired PCa tissues compared with the matched adjacent normal tissues as assessed by analyzing the TCGA PCa miRNA sequencing dataset. *P < 0.05. c miR-210-3p expression levels was markedly elevated in bone metastatic PCa tissues (PCa/BM) compared with that in non-bone metastatic PCa tissues (PCa/nBM) as assessed by analyzing the TCGA PCa miRNA sequencing dataset (PCa/nBM, n = 11; PCa/BM, n = 9).*P< 0.05. d Real-time PCR analysis of miR-210-3p expression in 81 non-bone metastatic and 68 bone metastatic PCa samples. Transcript levels were normalized to U6 expression. Lines represent median and lower/upper quartiles. *P < 0.05. e Real-time PCR analysis of miR-210-3p expression levels in normal prostate epithelial cell (RWPE-1), primary PCa cell 22RV1, bone metastatic PCa cell lines (PC-3, C4-2B and VCaP) and brain metastatic cell line DU145 and lymph node metastatic cell line LNCaP. Transcript levels were normalized to U6 expression. *P < 0.05