Fig. 4

miR-210-3p activates NF-κB signaling pathway. a NF-κB transcriptional activity was assessed by luciferase reporter constructs in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. b Western blotting of nuclear NF-κB/p65 expression. The nuclear protein p84 was used as the nuclear protein marker. c and d Real-time PCR analysis of TWIST1, MMP13 and IL11 in the indicated cells. Transcript levels were normalized to U6 expression. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. e NF-κB signaling inhibitors LY2409881 (10 μM) and JSH-23 (10 μM) attenuated the stimulatory effect of miR-210-3p on NF-κB transcriptional activity in the indicated cells respectively. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05 and **P < 0.01. f NF-κB signaling inhibitors LY2409881 (10 μM) and JSH-23 (10 μM) attenuated the stimulatory effect of miR-210-3p on invasion ability in the indicated cells respectively. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. g NF-κB signaling inhibitors LY2409881 (10 μM) and JSH-23 (10 μM) attenuated the stimulatory effect of miR-210-3p on migration ability in the indicated cells respectively. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05 and **P < 0.01