Skip to main content
Fig. 5 | Molecular Cancer

Fig. 5

From: Oncogenic miR-210-3p promotes prostate cancer cell EMT and bone metastasis via NF-κB signaling pathway

Fig. 5

miR-210-3p targets multiple negative regulators of NF-κB signaling. a Predictive target genes of miR-210-3p from TargetScan, miRanda and miRDB. b Western blotting of TNIP1, SOCS1, PIAS4 and PDLIM7 expression in the indicated cells. α-Tubulin served as the loading control. Protein expression levels of TNIP1, SOCS1, PIAS4 and PDLIM7 were quantified by densitometry using Quantity One Software, and normalized to the corresponding expression levels of α-tubulin respectively. The sample 1 was used as a standard. c and d Real-time PCR analysis of TNIP1, SOCS1, PIAS4 and PDLIM7 expression in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. e and f Luciferase assay of cells transfected with pmirGLO-3’UTR reporter of TNIP1 and SOCS1 in the miR-210-3p overexpressing and silencing VCaP and C4-2B cells. ​*P < 0.05. g and h MiRNP IP assay showing the association between miR-210-3p and TNIP1 and SOCS1transcripts in PCa cells cells. Pulldown of IgG antibody served as the negative control​ *P < 0.05.

Back to article page