Fig. 7

Clinical relevance of miR-210-3p with SOCS1, TNIP1 and NF-kB signaling activity in human PCa and bone tissues. a Analysis of miR-210-3p expression with SOCS1, TNIP1 and nuclear p65 in 3 bone metastatic PCa tissues, 3 non-bone metastatic PCa tissues and 3 metastatic bone tissues. U6 was used as the control for RNA loading. miR-210-3p expression levels were normalized to that miR-210-3p expression of sample one. Each bar represents the mean ± SD of three independent experiments. Loading controls were α-tubulin and p84 for the cytoplasmic and nuclear fractions. b Hypothetical model illustrating that constitutive activation of the NF-κB pathway by miR-210-3p epigenetic disruption of multiple negative feedback loops leads to bone metastasis of PCa