Fig. 3

Complex formation of hif-2α/β-catenin and its influence on each other. a Overexpression of hif-2α induced nuclear translocation of β-catenin in PANC-1 cells. b Co-existence of hif-2α and β-catenin in PANC-1 cells. c Interaction of hif-2α and β-catenin was noticed in PANC-1 cells. d The half-life of hif-2α was increased when β-catenin was up-regulated by LiCl. Cycloheximide was used to block protein biosynthesis. Densitometry was used to compare the degradation degree of the protein. e Increased β-catenin expression by LiCl enhanced HRE activity, and knockdown of β-catenin decreased HRE activity in normoxic or hypoxic conditions as indicated. *, P < 0.05; **, P < 0.01; ***, P < 0.001, as compared to pcDNA3, NaCl, or nc-siRNA. Representatives of two independent experiments