Multi-omics of 34 colorectal cancer cell lines - a resource for biomedical studies

Table 1 Mutation status in CRC critical genes. Cell lines were examined by Sanger sequencing, targeted sequencing or by both methods

	TP53	KRAS	BRAF	PIK3CA	PTEN	MSI	CIMP
CaCo2	p.E204X	wt	wt	wt	wt	MSS	CIMP-
CL-11^a	p.S215N	p.V14I; p.Q61H	wt	wt	wt	MSS	CIMP+
CL-34^a	p.S127P; p.K382fs	wt	p.V600E	wt	wt	MSI	CIMP+
CL-40^a	p.R248Q	p.G12D	wt	wt	wt	MSS	CIMP+
Co115	wt	wt	p.V600E	wt	p.E157fs; p.R233X	MSI	CIMP+
Colo205^b	p.Y107fs; p.Y103fs	wt	p.V600E	wt	wt	MSS	CIMP+
Colo320	p.R248W	wt	wt	wt	wt	MSS	CIMP-
Colo678^a	wt	p.G12D	wt	wt	wt	MSS	CIMP+
DLD-1	p.S241F	p.G13D	wt	p.E545K; p.D549N	wt	MSI	CIMP+
EB	wt	p.G12D	wt	p.E545K	wt	MSS	CIMP+
FRI	p.C277F	p.G13D	wt	p.E545K	wt	MSS	CIMP-
HCC2998^b	p.R213X	p.A146T	wt	wt	p.Y46C; p.R130Q; p.F341V	MSS	CIMP-
HCT116	wt	p.G13D	wt	p.H1047R	wt	MSI	CIMP+
HCT15	p.S241F	p.G13D	wt	p.E545K; p.D549N	wt	MSI	CIMP+
HT29	p.R273H	wt	p.V600E; p.T119S^c	wt	wt	MSS	CIMP+
IS1	p.Y163H	p.G12D	wt	wt	wt	MSS	CIMP-
IS3	p.Y163H	p.G12D	wt	wt	wt	MSS	CIMP-
KM12^b	p.P72fs; p.H179R	wt	p.P403fs	wt	p.G129X; p.K267fs	MSI	CIMP+
LoVo	wt	p.G13D; p.V14A	wt	wt	wt	MSI	CIMP-
LS1034	p.G245S	p.A146T	wt	wt	wt	MSS	CIMP-
LS174T	wt	p.G12D	p.D211G^c	p.H1047R	wt	MSI	CIMP-
NCI-H508	p.R273H	wt	p.G596R	p.E545K	wt	MSS	CIMP-
RKO	wt	wt	p.V600E	p.H1047R	wt	MSI	CIMP+
SW1116	p.A159D	p.G12A	wt	wt	wt	MSS	CIMP-
SW1463^a	p.R248Q	p.G12C	wt	wt	wt	MSS	CIMP-
SW403^a	p.E51X	p.G12V	wt	wt	wt	MSS	CIMP-
SW48	wt	wt	p.R347X^c	p.G914R^c	wt	MSI	CIMP+
SW480	p.R273H; p.P309S	p.G12V	wt	wt	wt	MSS	CIMP-
SW620	p.R273H; p.P309S	p.G12V	wt	wt	wt	MSS	CIMP-
SW837^a	p.R248W	p.G12C	wt	wt	wt	MSS	CIMP+
SW948	p.G117fs	p.Q61L	wt	p.E542K	wt	MSS	CIMP-
TC71	p.C176Y; p.R213X	p.G12D	wt	p.R88Q^c	p.R233X	MSI	CIMP-
V9P	p.G245D	wt	wt	wt	wt	MSS	CIMP-
WiDr	p.R273H	wt	p.V600E; p.T119S^c	wt	wt	MSS	CIMP+

All variants found with targeted sequencing and not observed in Sanger sequencing data were found in regions outside codons targeted by Sanger. HT29/WiDr had two variants each in BRAF, where one was verified by Sanger, and the other was outside of codons assessed. Only non-synonymous mutations were reported from targeted sequencing data
^aMutation data were available from Sanger sequencing only
^bMutation data were available from targeted sequencing only
^cMutations found with targeted sequencing only, but outside of regions assessed by Sanger sequencing

ISSN: 1476-4598