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Table 1 Mutation status in CRC critical genes. Cell lines were examined by Sanger sequencing, targeted sequencing or by both methods

From: Multi-omics of 34 colorectal cancer cell lines - a resource for biomedical studies

  TP53 KRAS BRAF PIK3CA PTEN MSI CIMP
CaCo2 p.E204X wt wt wt wt MSS CIMP-
CL-11a p.S215N p.V14I; p.Q61H wt wt wt MSS CIMP+
CL-34a p.S127P; p.K382fs wt p.V600E wt wt MSI CIMP+
CL-40a p.R248Q p.G12D wt wt wt MSS CIMP+
Co115 wt wt p.V600E wt p.E157fs; p.R233X MSI CIMP+
Colo205b p.Y107fs; p.Y103fs wt p.V600E wt wt MSS CIMP+
Colo320 p.R248W wt wt wt wt MSS CIMP-
Colo678a wt p.G12D wt wt wt MSS CIMP+
DLD-1 p.S241F p.G13D wt p.E545K; p.D549N wt MSI CIMP+
EB wt p.G12D wt p.E545K wt MSS CIMP+
FRI p.C277F p.G13D wt p.E545K wt MSS CIMP-
HCC2998b p.R213X p.A146T wt wt p.Y46C; p.R130Q; p.F341V MSS CIMP-
HCT116 wt p.G13D wt p.H1047R wt MSI CIMP+
HCT15 p.S241F p.G13D wt p.E545K; p.D549N wt MSI CIMP+
HT29 p.R273H wt p.V600E; p.T119Sc wt wt MSS CIMP+
IS1 p.Y163H p.G12D wt wt wt MSS CIMP-
IS3 p.Y163H p.G12D wt wt wt MSS CIMP-
KM12b p.P72fs; p.H179R wt p.P403fs wt p.G129X; p.K267fs MSI CIMP+
LoVo wt p.G13D; p.V14A wt wt wt MSI CIMP-
LS1034 p.G245S p.A146T wt wt wt MSS CIMP-
LS174T wt p.G12D p.D211Gc p.H1047R wt MSI CIMP-
NCI-H508 p.R273H wt p.G596R p.E545K wt MSS CIMP-
RKO wt wt p.V600E p.H1047R wt MSI CIMP+
SW1116 p.A159D p.G12A wt wt wt MSS CIMP-
SW1463a p.R248Q p.G12C wt wt wt MSS CIMP-
SW403a p.E51X p.G12V wt wt wt MSS CIMP-
SW48 wt wt p.R347Xc p.G914Rc wt MSI CIMP+
SW480 p.R273H; p.P309S p.G12V wt wt wt MSS CIMP-
SW620 p.R273H; p.P309S p.G12V wt wt wt MSS CIMP-
SW837a p.R248W p.G12C wt wt wt MSS CIMP+
SW948 p.G117fs p.Q61L wt p.E542K wt MSS CIMP-
TC71 p.C176Y; p.R213X p.G12D wt p.R88Qc p.R233X MSI CIMP-
V9P p.G245D wt wt wt wt MSS CIMP-
WiDr p.R273H wt p.V600E; p.T119Sc wt wt MSS CIMP+
  1. All variants found with targeted sequencing and not observed in Sanger sequencing data were found in regions outside codons targeted by Sanger. HT29/WiDr had two variants each in BRAF, where one was verified by Sanger, and the other was outside of codons assessed. Only non-synonymous mutations were reported from targeted sequencing data
  2. aMutation data were available from Sanger sequencing only
  3. bMutation data were available from targeted sequencing only
  4. cMutations found with targeted sequencing only, but outside of regions assessed by Sanger sequencing