Fig. 1

CASC15 expression is highest in acute leukemia samples that carried a translocation involving the RUNX1/AML1. a RT-qPCR of the B-ALL patient bone marrow samples using primer set #1 normalized to Actin, showing differential expression between cytogenetic subtypes based on translocations (n = 125). Comparisons were made using 1-way ANOVA (p < 0.02) and a two-tailed T-test; statistically significant differences are denoted as follows: P < 0.005 (**)). b RT-qPCR analysis of CASC15 expression in different molecular and cytogenetic subtypes of AML patient samples, using primer set #1 and normalized to GUS (n = 48). Comparisons were made using a two-tailed T-test. c Top: Schematic showing the genomic conservation of the syntenic block among vertebrates. Highly conserved region of the human CASC15 was used for the analysis. Bottom: Chip-seq histone modification map from the ENCODE/Broad institute, taken from UCSC genome browser, shows H3K4me3 and H3K36me3 patterns at the SOX4 and CASC15 locus in two different cell types indicating active transcription of the lncRNA