Fig. 2

Over-expression of CASC15 leads to increased apoptosis in B-ALL cells and myeloid expansion in mice. a RT-qPCR of mouse pre-B 70Z/3 cell line transduced with either human CASC15(s) or murine Casc15. b Annexin V staining of 70Z/3 stably transduced with CASC15(S) and mouse CASC15, either at baseline (DMSO) or treated with prednisolone (concentrations as indicated). c RT-qPCR or primary murine bone marrow cells transduced with murine Casc15. d Methylcellulose colony formation assay using MYC (positive control) or murine Casc15. e-f FACS analysis of peripheral bleed from mice 4 weeks after bone marrow transfer showing successful engraftment and transduction (GFP+) in vector (e) and CASC15 (f) over expression mice. g RT-qPCR showing Casc15 overexpression in bone marrow at 16 weeks after transplantation. h FACS analysis of peripheral bleeds from the mice 4–20 weeks after bone marrow transplantation showing increased percentage of myeloid cells in the Casc15 overexpression mice. i FACS analysis of peripheral blood showing percentage of B220+ and CD11b + cells at 16 weeks post-transplantation. j FACS analysis of bone marrow showing percentage of GFP+, B220+ and GFP+, CD11b + cells at 16 weeks post-transplantation. In experiments depicted for g-j, n = 8 mice/group, and experiments were repeated three times. Statistically significant differences are shown as follows: p < 0.05 (*); p < 0.01 (**); p ≤ 0.0005 (***). All qPCR analyses for CASC15 were performed with primer set #1 and normalized to GAPDH or actin, except where otherwise noted. Experiments were repeated three times for validation