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Fig. 4 | Molecular Cancer

Fig. 4

From: The lncRNA CASC15 regulates SOX4 expression in RUNX1-rearranged acute leukemia

Fig. 4

Casc15 knockout in murine cells results in Sox4 downregulation. a Schematic of Casc15 gene in the mouse showing exonic sequences 1 and 2, along with the placement of short guide RNAs to induce deletion of these exons. b Schematic of approach to generation of genomic-level knockouts of Casc15. c FACS analysis of mCherry expression ion bulk transductants from this approach. d PCR analysis of mutant (DEL) and wild-type (WT) Casc15 alleles in bulk transductants cells. e FACS analysis of mCherry expression in single cell clones generated by limiting dilution plating. f PCR analysis of mutant (DEL) and wild-type (WT) Casc15 alleles in single cell clones generated by limiting dilution plating. g RT-qPCR analysis of Casc15 and Sox4 expression. Statistically significant differences from control are noted as follows: p < 0.05 (*); p < 0.01 (**)

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