Fig. 6

CASC15 regulates the activity of YY1 on the SOX4 promoter. a Transcriptional activity of SOX4 promoter upon CASC15 (L) and/or YY1 overexpression, as measured by dual luciferase assay. Luciferase values are normalized to the empty vector. b-d RT-qPCR with primers directed against SOX 4 (b) CASC15 (c) and YY1 (d) and Western blot for YY1 (e) in 293 T cells transiently transfected with YY1 and CASC15. All qPCR analyses for CASC15 were performed with primer set #1 and normalized to GAPDH or actin, except where otherwise noted. Experiments were repeated three times for validation