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Fig. 1 | Molecular Cancer

Fig. 1

From: Exosomes derived from gemcitabine-resistant cells transfer malignant phenotypic traits via delivery of miRNA-222-3p

Fig. 1

Gemcitabine-resistant cells shed exosomes that readily enter A549-P cells. a A549-P/GR cells were treated with increasing concentrations of gemcitabine for 72 h. Cell growth was analyzed by MTS assay. Data represent at least three experiments performed in triplicate. Error bars represent ± SD. *P < 0.01; **P < 0.001. b A549-P/GR cells were treated with gemcitabine (1 μM) and examined by colony formation assay. c The presence of TSG101, CD81, and Alix in 15 μg of exosomes was analyzed by western blot. d Exosomes were observed by transmission electron microscopy. Scale bar, 100 μm. e Quantification of exosomes expelled from equal numbers of A549-P/GR cells by BCA analysis. f A549-P cells were treated with different pharmacological inhibitors of endocytic pathways for 2 h. A549-GR–derived exosomes (GR-Exo) were dyed with PKH67 (green) and incubated with pretreated cells for 4 h, and exosome uptake was viewed under confocal microscopy

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