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Fig. 2 | Molecular Cancer

Fig. 2

From: Exosomes derived from gemcitabine-resistant cells transfer malignant phenotypic traits via delivery of miRNA-222-3p

Fig. 2

GR-Exo deliver miRNA-222-3p into recipient cells, enhance gemcitabine resistance, promote cell metastasis and proliferation. a Heatmap of differential miRNA expression between A549-GR and A549-P exosomes. Gene expression data were obtained using a human microRNA array. Expression values shown are mean centered. Red: increased expression, Green: decreased expression. b miR-222-3p expression was detected in A549-P/GR cells and A549-P/GR–derived exosomes by qRT-PCR. c miR-222-3p expression was detected in A549-P cells after co-incubation with P-Exo or GR-Exo for 24 h by qRT-PCR. d miR-222-3p expression was detected in H460 and H157 cells after co-incubation with P-Exo or GR-Exo by qRT-PCR. e The growth rates of A549-P and A549-P-KD cells in the absence or presence of GR-Exo were assessed using MTS assay. f Cell growth was assessed by MTS after treatment with gemcitabine for 72 h in the absence or presence of P-Exo or GR-Exo. g The migration of exosome-treated cells was assessed using wound-healing assay. Right, quantitative analysis of invasive cells. h Transwell assay was performed to assess cell invasion. Right, quantitative analysis of scratch wound closure. i Soft agar assay was performed to examine the anchorage-independent survival of cells. Right, quantitative analysis of cell clones. Data represent at least three experiments performed in triplicate. *P < 0.05; **P < 0.01

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