Fig. 4

Exogenous overexpression of SOCS3 rescues A549-P cells from GR-Exo–induced stronger malignancy. A549-P or A549-GR cells were transfected with GV-144-SOCS3 plasmid and treated with GR-Exo. a SOCS3 expression was assessed by western blotting. b Cell viability was assessed by MTS after co-treatment with gemcitabine for 72 h. Data are representative of three experiments. Error bars represent ±SD. *P < 0.05; **P < 0.01 vs. control. c Wound-healing assay and d Transwell assay were performed to assess cell migration and invasion. Lower, quantitative analysis of scratch wound closure (c) or cell invasion (d). Data represented at least three experiments performed in triplicate. *P < 0.05; **P < 0.01