Fig. 5

Knockdown of miR-222-3p in A549-P/GR cells suppresses tumor growth and lung metastasis in vivo. Lung metastasis models in SCID mice were generated by using luciferase-labeled A549-P/GR cells with lentivirus miR-222-3p KD or normal control (NC) and treated with PBS (control) or GR-Exo (n = 8). a miR-222-3p expression was assessed by qRT-PCR. b SOCS3 expression was assessed by western blotting. Two samples were selected from each group randomly (n = 8) (labeled as 1,2), and all samples were sufficiently mixed as a pool (labeled as mix). c The incidence of metastasis in the lung or other organs in each group. d Luminescence was assessed in 8 mice of each group at 6 weeks after injection. e To quantify the metastatic mass, the photon counts per second were recorded (***P < 0.001). f The lungs of mice from each group were removed, and lung metastasis was evaluated by hematoxylin and eosin (H&E) staining