Fig. 2

MiR-661 promoted invasion and metastasis of NSCLC. a. Wound healing assay of A549 with miR-661 overexpression or anti-miR-661 expression at 0 h, 24 h and 48 h after transfection. Migration distance (μm) was calculated by subtracting the distance at 24 h or 48 h from that at 0 h. Migration ability was expressed by migration index-the distance migrated by treated cells relative to that by control cells. b. Wound healing assay of SPC-A1 with miR-661 overexpression or anti-miR-661 expression at 24 h and 48 h after transfection. Migration ability was expressed by migration index. c. Transwell assay of A549 and SPC-A1 with increased miR-661 expression. d. Transwell assay of A549 and SPC-A1 with miR-661 inhibitor (anti-miR-661). e. H&E staining of lung nodules formed after injection of A549 carrying miR-661 (LV-miR-661) and empty vector (LV-NC) through tail vein. f. Comparison of numbers of metastatic nodules formed after caudal vein injection of A549 carrying LV-miR-661 and LV-NC. All experiments were performed in triplicates. Error bars are mean (n = 3) ± SEM (* P < 0.05, ** P < 0.01) in panel a, b, c and d