Fig. 3
From: Metformin suppresses cancer initiation and progression in genetic mouse models of pancreatic cancer
Metformin suppressed chronic pancreatitis-associated tumorigenesis. a Schematic presentation of the induction of chronic pancreatitis and treatment of metformin in KC mice. b Macroscopic picture, histology and IHC staining of CK19 in mice treated with vehicle, cerulein, or cerulein plus metformin. Scale bars: H&E (top row) = 400 μm; (2nd row) = 100 μm; CK19 (top row) = 400 μm; (2nd row) = 100 μm. c Quantification of the weight of the pancreas. d Statistical analysis of the CK19-positive area. (e) Quantification of the percentages of early pancreatic lesions (ADM plus mPanIN1), late mPanIN lesions (ADM plus PanIN1) and PDAC in mice treated with vehicle, cerulein, or cerulein plus metformin. nd, not detected. *P < 0.05; **P < 0.01