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Fig. 5 | Molecular Cancer

Fig. 5

From: Role of MEK partner-1 in cancer stemness through MEK/ERK pathway in cancerous neural stem cells, expressing EGFRviii

Fig. 5

LAMTOR3 expression and ERK activation in F3.EGFRviii cells. a MP1 expression was determined by immunoblot analysis of F3.EGFRviii cells transduced with a shRNA control lentivirus (shCtl) or a lentivirus expressing shRNA for LAMTOR3 (shLAMTOR3 #1 and #2), using α-tubulin as a loading control. b The levels of pERK1/2 and pAKT were determined by immunoblotting in F3.EGFRviii cells. Total ERK2 was used as a loading control. c and d F3.EGFRviii cells were treated with PMA (l μg/ml) and harvested at the indicated times for immunoblot analysis using β-actin as a loading control

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Molecular Cancer

ISSN: 1476-4598