Fig. 1
From: EGFR modulates monounsaturated fatty acid synthesis through phosphorylation of SCD1 in lung cancer
EGFR interacts with SCD1. a and b Lysates from 293Â T cells exogenously expressing EGFR-HA (at C-terminus) and Flag-SCD1 (at N-terminus) were subjected to immunoprecipitation (IP) and immnuoblotting (IB) with the indicated antibodies. WCL, whole cell lysates. c Reciprocal immunoprecipitation and western blot analysis in HCC827 cells. Ten percentage of the lysate for immunoprecipitation is shown as input. d and e Lysates from 293Â T cells exogenously co-expressing Flag-SCD1 and full-length or different functional domains of EGFR-HA (d), or co-expressing EGFR-HA and full-length or different regions of Flag-SCD1 (e) were subjected to IP/IB with the indicated antibodies. FL, full-length