Fig. 4
From: EGFR modulates monounsaturated fatty acid synthesis through phosphorylation of SCD1 in lung cancer
Phosphorylation of Y55 is necessary for maintainingSCD1 protein stability. a-d A549 cells were infected with SCD1 shRNA-2 lentivirus which specifically knocked down endogenously expressed SCD1 and then transfected with empty vector, ectopic wild-type SCD1 and different mutants, respectively. a The lysates were immunoblotted with the indicated antibodies. Y55 phosphorylation of SCD1 was examined by IP/IB. b A549 transfectants were incubated with/without MG132 (10 μM) for 12 h and the lysates were then subjected to IB. c A549 transfectants were treated with CHX (100 μg/ml) for the indicated time. The lysates were then subjected to IB. Densitometry quantitative data (Flag/Tubulin) are mean ± SEM from three independent experiments. d A549 transfectants were incubated with or without MG132 (10 μM) for 12 h. Then, Flag-tagged SCD1 was immunoprecipitated and blotted with an anti-ubiquitin antibody. Densitometry quantification of Flag/Tubulin (a and b) levels is shown