Fig. 5
From: EGFR modulates monounsaturated fatty acid synthesis through phosphorylation of SCD1 in lung cancer
Phosphorylation of Y55 is required for SCD1 to promote lung cancer growth. a Total cell lipids were extracted and the ratio of monounsaturated fatty acids (18:1) to saturated fatty acid (18:0) was determined by LC-MS in A549 stable cell lines. Data are mean ± SD (n = 3). b Proliferation rates of A549 transfectants were examined by CCK-8 assay in vitro. Data are mean ± SD (n = 5). c Cell cycle of A549 stable cell lines was detected by propidium iodide (PI) staining. The percentage of cells in G1 phase or S/G2/M phase is shown. d Cell apoptosis of A549 transfectants was determined by Annexin V/PI staining assay. e In vitro growth of A549 stable cell lines was assessed by colony formation assay. The representative pictures of developed colonies are shown and the number was counted after 9 days. Data are mean ± SD (n = 3). f and g A549 transfectants were subcutaneously injected into nude mice and tumor volume was measured. Tumor growth curves and tumor sizes are shown. Data are mean ± SEM (n = 8). ***p < 0.001; **p < 0.01; *p < 0.05