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Fig. 2 | Molecular Cancer

Fig. 2

From: Downregulation of NMI promotes tumor growth and predicts poor prognosis in human lung adenocarcinomas

Fig. 2

NMI inhibited clone formation and induced apoptosis in lung cancer cells. a Colony formation assay and quantitative analysis were performed with in A549 and H460 cells with NMI overexpression or knockdown. b FACS analysis was used to determine the relative percentage of apoptotic cells in A549 and H460 cells. c Western blot and quantitative analysis were used to detected the expression of Bcl-2, cleaved caspase-3/9 and phosphorylated PARP proteins in A549 and H460 cells at 48 h after transfection with NMI or control plasmids. d Western blot and quantitative analysis were used to detected the levels of phosphorylated PI3K, AKT, ERK, PKDK1, GSK-3β, p110β and p38 proteins in A549 and H460 cells at 48 h after transfection with NMI-shRNAs or control. e Western blot and quantitative analysis were used to detected the expression of the phosphorylated PI3K, AKT, pDK1, GSK-3β and p110β proteins in A549 and H460 cells at 48 h after transfection with NMI overexpression or control plasmids

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