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Fig. 4 | Molecular Cancer

Fig. 4

From: Downregulation of NMI promotes tumor growth and predicts poor prognosis in human lung adenocarcinomas

Fig. 4

NMI regulated transcriptional activation of COX-2 and PGE2 production. a Schematic diagram of 6 different fragments of COX-2 promoter. b The activity of different fragments of COX-2 promoter was determined by luciferase reporter assay performed in A549 cells transfected with the control LacZ plasmid or the NMI overexpression plasmid. c, d Western blot and quantitative analysis were used to detected the expression of COX-2 in A549 and H460 cells with NMI overexpression (C) or NMI knockdown (D). e The PGE2 production level was measured in A549 and H1299 cell culture media at 72 h after transfection. f Cell viability was determined by MTT assay in A549 and H1299 cells

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