Fig. 3

miR-140-5p inhibits GC cell proliferation, migration and invasion in vitro. a Effect of miR-140-5p on GC cell growth determined by MTT assays. b Colony formation assays in GC cells transfected with miR-140-5p or control mimics. Data are representative of at least three independent experiments; values are expressed as means ± standard errors of the means (SEMs). c Wound healing assays showing the inhibition of miR-140-5p on the migratory abilities of GC cells. d Transwell assays in GC cells transduced with miR-140-5p or control mimics. Data are representative of at least three independent experiments; values are expressed as means ± SEMs. e Western blot analysis of markers associated with tumor proliferation, migration, and invasion, as well as SOX4, after miR-140-5p transfection in vitro