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Fig. 4 | Molecular Cancer

Fig. 4

From: MicroRNA-218 functions as a tumor suppressor in lung cancer by targeting IL-6/STAT3 and negatively correlates with poor prognosis

Fig. 4

Inhibition of IL-6/JAK3/STAT3 signaling reduces cell proliferation and colony formation in vitro. a A549 and H1795 cells were transfected with siR-IL-6R, or siR-JAK3. Twenty-four hours after transfection, cells were lysed and IL-6R, JAK3, pSTAT3 and GAPHD were analyzed by western blotting. b-d A549 and H1795 cells were transfected with siR-IL-6R (b) or siR-JAK3 (c) or were treated with 1 μL of LLL-12 (d). Seventy-two hours after transfection or treatment, cells were counted. The data shown were the means ± SD of three individual experiments. e-g A549 and H1795 cells were transfected with siR-IL-6R (e) or siR-JAK3 (f) or were treated with 1 μL of LLL-12 (g). Ten days after transfection or treatment, cells were fixed and stained by crystal violet

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