Fig. 5

MiR-218 regulates the survival of ALDH positive lung cancer cells through STAT3 signaling. a ALDH1A1 positive cells were isolated from H1975 and A549 cells and the levels of miR-218 were measured by real time PCR. b The levels of IL-6R, JAK3 and pSTAT3 in ALDH1A1 positive cells were analyzed by western blotting. c ALDH1A1 positive cells were infected by lentivirus expressing miR-218 or miR-control. The levels of miR-218 in these infected cells were examined by real time PCR. d The levels of IL-6R, JAK3, phosphorylated STAT3, STAT3 and GAPDH in the infected cells were determined by western blotting. e The ALDH1A1 positive cells were plated as single cells in ultra-low attachment six-well plates and were cultured in a serum-free MEBM. Twenty-four hours after seeding, the ALDH1A1 positive cells were infected with lentivirus expressing miR-218 or miR-control