Fig. 1

Gene structures and expressions of ZEB1-AS1 and ZEB1. a, schematic diagram of ZEB1-AS1 and ZEB1 structures. ZEB1-AS1 (upstream, red) was transcribed from the promoter region of ZEB1 (downstream, green), with a conversed orientation of ZEB1. The full length of ZEB1-AS1 was 2535 nt, including two exons (E1, 167 nt; E2, 2013 nt) and one intron (355 nt). b, the full sequence of ZEB1-AS1. Nucleotides labeled by black color were exons and those of gray were intron. c, RNA levels of ZEB1-AS1 and ZEB1 in a series of cell lines. RT-PCR analysis (with GAPDH as control) showed expression levels of ZEB1-AS1 and ZEB1 in benign prostate hyperplasia sample BPH, normal prostate epithelial cell RWPE-1, prostate cancer (PC3, DU145), glioma (T98G, U251), melanoma (A375, A875), pulmonary cancer (A549, 1299, SK-MES-1), cervical cancer (HeLa), 293, HUVEC, liver cancer (LO2) and normal human bronchial epithelial cell (HBE) (upper). Grey value analysis showed synchronous expression levels of ZEB1-AS1 and ZEB1 (lower). d, RNA levels of ZEB1-AS1 in prostate cancer specimens. Q-PCR analysis was used to detect the expression of ZEB1-AS1 in 9 pairs of fresh prostate cancer specimen (PCa) and the corresponding paracancerous tissue (paraCa). There was a significant difference between the two groups