Fig. 3

Effects of ghrelin and In1-ghrelin on PCa pathophysiological processes. a. Cell proliferation of PCa cell lines after treatment with ghrelin or In1-ghrelin derived peptides for 4-24 h (10 nM; n ≥ 3). Treatment with IGF-1 and PCX were used as positive and negative controls, respectively. b. Effect of ghrelin and In1-ghrelin peptides treatment on the migration PC-3 cell line was determined by wound healing assay (12 h; n ≥ 3). Representative images showing the higher migration capacity of PC-3 cells after treatment with In1-ghrelin peptides are depicted. c. phospho-ERK and phospho-AKT time-course activation after treatment with ghrelin or In1-ghrelin peptides (5–30 min) in LNCaP and PC-3 cell lines. Protein levels of phospho-ERK and phospho-AKT were adjusted by total ERK and AKT, respectively. Data represent mean ± SEM. Asterisks (***p < 0.001, **p < 0.01, *p < 0.05) indicate differences between In1–19 and vehicle-treated controls, and dashes between In1–40 and vehicle control treatment (# < 0.05; ##p < 0.01). Representative blots in LNCaP cell line are showed