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Fig. 5 | Molecular Cancer

Fig. 5

From: The oncogenic role of the In1-ghrelin splicing variant in prostate cancer aggressiveness

Fig. 5

Gene expression effects of ghrelin and In1-ghrelin overexpression in PC-3 and derived xenografted tumors. a. Results from the RT2 Prostate-Cancer PCR Array which evaluates the expression of 84 genes involved in prostate cancer development and progression performed in ghrelin and In1-ghrelin-stably transfected PC-3-cells compared with control-mock PC-3-cells. The graphs indicate those genes which expression change ≥ 1.5-fold; b. Validation by qPCR of genes dysregulated in the RT2 Prostate-Cancer PCR Array using different cell preparations (n ≥ 3) and new sets of primers; c. Validation by Western blot of the changes observed in the previous analysis; d. Expression of angiogenic factors in In1-ghrelin-stably transfected PC-3-cells and native ghrelin-stably transfected PC-3 cells compared with control-mock PC-3-cells.; e. Expression of angiogenic factors in xenografted tumors of stably transfected-PC-3 cells. f. CAV1, LOXL1, SFRP1 and IL-6 mRNA expression levels in mock, ghrelin and In1-ghrelin transfected PC-3-derived xenografted tumors. Results were normalized with ACTB. All preparations were repeated at least three times (n ≥ 3). (*p < 0.05; **p < 0.01; ***p < 0.001). Values represent mean (±SEM) or median (IQR). Asterisks (***p < 0.001; **p < 0.01; *p < 0.05) indicate values that significantly differ from the mock control

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