Fig. 6
From: The oncogenic role of the In1-ghrelin splicing variant in prostate cancer aggressiveness
Effects of In1-ghrelin silencing on PCa cell proliferation and PSA secretion. a. Validation by qPCR of In1-ghrelin silencing in PC-3; b. Validation by qPCR of In1-ghrelin silencing in LNCaP cells. In both cases, expression levels were adjusted by a normalization factor (NF) calculated from ACTB and GAPDH expression levels; c. Proliferation rates of In1-ghrelin-silenced PC-3 and LNCaP cells compared with control scramble-transfected cells; d. PSA secretion of In1-ghrelin-silenced LNCaP cells compared with control scramble-transfected cells. All experiments were repeated at least three times (n ≥ 3). Data were evaluated by two-tailed t-test to analyze significant differences (*p < 0.05; **p < 0.01, ***p < 0.001) and represent mean ± SEM and are expressed as percentage vs control (scramble-treated cells), which was set at 100%