Fig. 4

Identification of exosomal lncRNA-UCA1 in normoxic and hypoxic exosomes derived from 5637 cells. a Schematic representation of the UCA1 gene structure and the designed primers used for our study are shown in this schematic diagram. b and c Reverse transcription-PCR (RT-PCR) analysis of the full-length and fragments of lncRNA-UCA1 in normoxic and hypoxic exosomes derived from 5637 cells. d and e Quantitative real-time PCR (qRT-PCR) analysis of lncRNA-UCA1 expression in normoxic and hypoxic exosomes derived from 5637 cells. The samples were untreated with or treated with RNase A (10 μg/ml) and/or 0.3% Triton X-100 and then further mixed with of RNase inhibitor (mean ± S.E.M., *P < 0.05, n = 3, NS, not significant). f and g qRT-PCR analysis of lncRNA-UCA1 expression in UMUC2 cells co-cultured with different concentrations of normoxic and hypoxic exosomes derived from 5637 cells (mean ± S.E.M., *P < 0.05, n = 3). h and i qRT-PCR analysis of lncRNA-UCA1 expression in normoxic and hypoxic 5637 cells and normoxic and hypoxic exosomes derived from 5637 cells (mean ± S.E.M., *P < 0.05, n = 3)