Fig. 6

Hypoxic exosomal lncRNA-UCA1 promotes tumor growth and progression in vivo. a ~ d Effect of hypoxic exosomes derived from 5637 cells transfected with control shRNA or UCA1 shRNA on tumor growth in a xenograft model. Tumor volume and body weight of xenograft models were measured from day 0 to day 18, and final tumor weights were determined. (mean ± S.E.M., *P < 0.05, n = 6). e qRT-PCR analysis of lncRNA-UCA1 expression in xenograft tumor tissues treated with PBS or hypoxic exosomes derived from 5637 cells transfected with control shRNA or UCA1 shRNA (mean ± S.E.M., *P < 0.05, n = 3). f qRT-PCR analysis of lncRNA-UCA1 expression in 5637 cells co-cultured with PBS or hypoxic exosomes derived from 5637 cells transfected with control shRNA or UCA1 shRNA (mean ± S.E.M., *P < 0.05, n = 3). g Western blotting analysis of Ki67, E-cadherin, vimentin and MMP9 protein levels in 5637 cells co-cultured with PBS or hypoxic exosomes derived from 5637 cells transfected with control shRNA or UCA1 shRNA. h Hematoxylin and eosin-stained images and immunohistochemistry analysis of E-cadherin and MMP9 protein levels in xenograft tumor tissues (scale bar: 100 μm)