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Fig. 6 | Molecular Cancer

Fig. 6

From: Up-regulation of lncRNA CASC9 promotes esophageal squamous cell carcinoma growth by negatively regulating PDCD4 expression through EZH2

Fig. 6

CASC9 regulated PDCD4 via EZH2. a Knockdown of EZH2 increased the expression of PDCD4, which was detected by Western blotting. b RNA-protein pull down indicated the binding of CASC9 with EZH2. c RIP showed that CASC9 was significantly enriched with EZH2 antibody compared with lgG in KYSE150 and KYDE450 cells. d CACS9 was distributed in both nucleus and cytoplasm analyzed by FISH CASC9 was labeled by FITC (green) and U6 was labeled by Cy3 (red). Nucleus was stained by DAPI (blue). The ESCC tissue sections were observed in 200X magnification to show a complete keratin pearl. The slides of ESCC cells were observed in 630X magnification. e CACS9 was distributed in both nucleus and cytoplasm analyzed by subcellular fractionation assay. f Knockdown of CASC9 decreased the enrichment of EZH2 and H3K27me3 in the PDCD4 promoter region in ESCC cells (KYSE150 and KYSE450). Data were mean ± SD. *P < 0.05, **P < 0.01,***P < 0.001

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Molecular Cancer

ISSN: 1476-4598