Fig. 3

Validation of LATS1 as a target gene of miR-424 in GC cells. a KEGG enrichment analysis of the association of miR-424 expression with enriched signaling pathways by using mirPath v.3 software. b Relative expression levels of miR-424 and LATS1 in different GC cell lines and GES-1 cells and spearman correlation analysis of their correlation in GC cells. c and d qRT-PCR and western blot analysis of the expression levels of miR-424 and LATS1 after transfection with miR-424 mimic or inhibitor in HGC-27 or MKN28 cell lines. e The binding sites of wild type or mutant LATS1 3’UTR with miR-424. f and g The luciferase activity of wild type LATS1 3’UTR or mutant LATS1 3’UTR after transfection with miR-424 mimic or inhibitor in HGC-27 or MKN28 cell lines. Data are the means ± SEM of three experiments. **P < 0.01