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Fig. 7 | Molecular Cancer

Fig. 7

From: Circular RNA_LARP4 inhibits cell proliferation and invasion of gastric cancer by sponging miR-424-5p and regulating LATS1 expression

Fig. 7

circLARP4 acts as a miRNA sponge for miR-424 in GC cells. a Schematic representation of potential binding sites of miRNAs with circLARP4. b The luciferase activity of circLARP4 after transfection with pMIR-Luc-circLARP4 combined with each miRNA mimic in HEK-293 T cells. c1 The effects of circLARP4 overexpression of knockdown on the expression level of miR-424 in HCG-27 or MKN-28 cell line indicated by qRT-PCR. c2 The luciferase activity of wild type circLARP4 3’UTR or mutant circLARP4 3’UTR after transfection with miR-424 mimic or inhibitor in HGC-27 or MKN-28 cell lines. d AGO2 RNA immunoprecipitation (RIP) assay for the amount of circLARP4 and miR-424 in HGC-27 or MKN-28 cells. Ago2 was detected using IP-western blot (up panel), and circLARP4 and miR-424 expression levels were detected using qRT-PCR (down panel). e Western blot analysis of the expression levels of YAP and p-YAP (S127) after transfection with circLARP4 + miR-424 in HGC-27 cells or si-circLARP4 + miR-424 inhibitor in MKN-28 cells. f MTT analysis of cell proliferation of HGC-27 or MKN-28 cells after transfection with circLARP4 + miR-424 or si-circLARP4 + miR-424 inhibitor. Data are the means ± SEM of three experiments. *P < 0.05; **P < 0.01

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