Fig. 2From: A novel mechanism of lncRNA and miRNA interaction: CCAT2 regulates miR-145 expression by suppressing its maturation process in colon cancer cellsLocalization of CCAT2 in CR-HT-29 or HCT-116 cells by qRT-PCR and FISH. qRT-PCR was performed with RNA isolated from nuclear and cytoplasmic fraction of stably over-expressing CCAT2 cells to determine its expression in CR-HT-29 and HCT-116 cells (a and b). All the data represent means ± SEM, *P < 0.001, compared to the control. (c) The representative photograph showing fluorescence in situ hybridization in CCAT2 knockout or overexpressing HCT-116 cells, red represents biotin-labeled probe against CCAT2 RNA and blue represents DAPI staining of the nucleusBack to article page