Fig. 2
From: Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes
In vitro binding of selected Id-peptides to the 5T33MM-Ig. a Concentration-dependent binding of N-biotinylated synthetic Id-peptides to purified 5T33MM-Ig as determined by ELISA. Peptide binding to A20 secreted Igs and polyclonal mouse Igs were included as controls. b Concentration-dependent binding of FITC-conjugated p5 peptide and relative control peptide to 5T33MM cells, as measured by flow cytometry. c Representative confocal images of 5T33MM cells labeled with FITC-conjugated p5 peptide (green), stained with the APC-conjugated anti-mouse IgG antibody (white), and DAPI (blue). The analysis was performed using a Leica TCS SP2 confocal microscope at 40X magnification