Fig. 3
From: Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes
In vitro characterization of exosome preparations. a Scanning electron microscopy (SEM). The 5T33MM cell culture supernatant was filtered (0.22 μm) and the exosomes were purified using the ExoQuick-TC™. The SEM analysis was performed at 20, 000 x magnification (SEM FEI Novalab 600). b Size distribution of the exosomes population derived from 5T33MM cells using dynamic light scattering (Zetasizer Nano S, Malvern Instruments). c B-cells from the blood of multiple myeloma patient and 5T33MM cells as well as purified exosomes from patient serum or 5T33MM cell supernatant were lysed in RIPA buffer, separated by SDS-PAGE, and analyzed by Western blotting using the indicated antibodies