Fig. 4
From: Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes
Flow cytometry of purified exosomes derived from cultured B-cells. Exosomes were purified from supernatants of the murine 5T33MM and human IM9 multiple myeloma cells, and the murine A20 B-lymphoma cells. The production of IgG-bearing exosomes was similar in the three cell lines. The p5 peptide recognized specifically the 5T33MM-released exosomes. Front/side scattering of exosome-bound SMNPs (External plot); Exo-Red-stained exosomes incubated with FITC-conjugated anti-IgG (left plots); Exo-Red-stained exosomes incubated with FITC-conjugated p5 peptide (central plots); Exo-Red-stained exosomes incubated with FITC-conjugated pCNT (right plots)