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Fig. 4 | Molecular Cancer

Fig. 4

From: Reprogramming of stromal fibroblasts by SNAI2 contributes to tumor desmoplasia and ovarian cancer progression

Fig. 4

SNAI2 transforms normal fibroblasts to a CAF-like state. a and b Representative images and quantification of the cellular cytoskeleton by F-actin staining and SNAI2 immunofluorescence in the MRC5 cells (a). and the ovarian normal fibroblasts (NFs) (b). after transfection with Ade-ctrl or Ade-SNAI2. The nuclei were counterstained with DAPI. c Western blot analysis of PDGFRB, FAP, ACTA2 and SNAI2 in the MRC5 cells and the primary ovarian NFs after a 72 h transfection with Adeno-ctrl or Adeno-SNAI2. GAPDH served as the loading control. d Representative images and quantification of the collagen contraction capacity of the MRC5 cells and the primary ovarian NFs in the Adeno-ctrl and Adeno-SNAI2 transfection group. e Representative images and the quantification of the proliferation index of SKOV3 cells cocultured with MRC5 cells and primary ovarian NFs in the Adeno-ctrl and Adeno-SNAI2 transfection group (**P < 0.01, ***P < 0.001)

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