Fig. 5

SNAI2 Drives a Transcriptional Program in the mesenchymal OC subtype that predicts clinical outcome. a Graphical depiction of the designation of the SNAI2 mesenchymal signature through overlapping genes significantly correlated with snai2 expression in the TCGA mesenchymal and Tothill C1 subtype. b and c Single sample GSEA (ssGSEA) analysis showing the relative expression of the SNAI2 mesenchymal signature score in various subtypes included in the Tothill’s dataset (b) and the ovarian TCGA dataset (c). d–f ssGSEA analysis showing the relative expression of the SNAI2 mesenchymal signature in NFs and CAFs in the stroma profile of OC (GSE0595) (d), breast cancer (GSE9014) (e) and colon cancer (GSE35602) (f). g and h Top ten most enriched GO annotation (g) and five most enriched KEGG pathways (h) in the set of the 77 genes included in SNAI2 mesenchymal signature. Count: number of genes that are involved in a given pathway. The fold enrichment shows how many fold more a given term was overrepresented in the 77 genes compared with a background of the total human genome. i STRING (Search Tool for the Retrieval of Interacting Genes) analysis of the proteins included in the SNAI2 mesenchymal signature, with the ECM network central in the network. Lines represent associations between proteins, and the line thickness reflects the number lines of evidence for each association. The dashed circles indicate the proteins included in the collagen family. j–l Kaplan-Meier analysis of the SNAI2 mesenchymal signature score expression in the patients included in three independent sets of the OC as a Tothill’s dataset (GSE9891) (j), Bonome’s dataset (GSE26712) (k) and Karlan’s dataset (GSE51088) (l). Kaplan-Meier curves were performed using the log-rank test. (*P < 0.05, ***P < 0.001)