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Fig. 1 | Molecular Cancer

Fig. 1

From: LncRNA RP11-436H11.5, functioning as a competitive endogenous RNA, upregulates BCL-W expression by sponging miR-335-5p and promotes proliferation and invasion in renal cell carcinoma

Fig. 1

LncRNA RP11-436H11.5 expression is upregulated in RCC tissues and promotes RCC cell proliferation and invasion. a. A qRT-PCR assay was performed to screen 14 potential lncRNAs. LncRNA RP11-436H11.5 was the most appropriate lncRNA to select. Each column is the ratio of caner tissue / normal tissue. b. LncRNA RP11-436H11.5 expression in RCC tissues vs normal renal tissues. c. Association of lncRNA RP11-436H11.5 expression with OS analysis of 20 RCC patients. P values are given on the figures. d and e. Verification of lncRNA RP11-436H11.5 knockdown (d) and overexpression (e) by qRT-PCR assay. f and g. A498 and 786-O cells were transfected with lncRNA RP11-436H11.5-shRNA (f) and functional lncRNA RP11-436H11.5-cDNA (g). Cell growth was measured by a cell proliferation assay. h and i. A chamber-transwell invasion assay was performed using A498 (h. pLVTHM vs shlncRNA RP11-436H11.5) and 786-O (i. pLVTHM vs oelncRNA RP11-436H11.5) cells. The invaded cells were counted in 10 randomly chosen microscopic fields (100×) of each experiment and pooled. d, e, f, g, h and i. Each sample was run in triplicate and in multiple experiments for mean ± SEM. *P < 0.05, **P < 0.01 compared to controls

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Molecular Cancer

ISSN: 1476-4598