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Fig. 3 | Molecular Cancer

Fig. 3

From: LncRNA RP11-436H11.5, functioning as a competitive endogenous RNA, upregulates BCL-W expression by sponging miR-335-5p and promotes proliferation and invasion in renal cell carcinoma

Fig. 3

LncRNA RP11-436H11.5 regulates BCL-W expression via miR-335-5p. a. A qRT-PCR assay was performed to detect the expression of BCL-W after knocking down lncRNA RP11-436H11.5 in A498 and 786-O cells. b. An argonaute2 immunoprecipitation assay was performed after transfecting with lncRNA RP11-436H11.5-shRNA in A498 and 786-O cells. c and d. A qRT-PCR assay was performed to detect the expression of miR-335-5p after knocking down lncRNA RP11-436H11.5 in A498 cells (c) and overexpressing lncRNA RP11-436H11.5 in 786-O cells (d). e. Correlation analysis of miR-335-5p and lncRNA RP11-436H11.5 by Pearson’s correlation coefficient. f. miR-335-5p level was measured in patients with low lncRNA RP11-436H11.5 (n = 10) and high lncRNA RP11-436H11.5 (n = 10) expression. a, b, c and d. Each sample was run in triplicate and in multiple experiments for mean ± SEM. *P < 0.05, **P < 0.01 compared to controls

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Molecular Cancer

ISSN: 1476-4598