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Fig. 5 | Molecular Cancer

Fig. 5

From: LncRNA RP11-436H11.5, functioning as a competitive endogenous RNA, upregulates BCL-W expression by sponging miR-335-5p and promotes proliferation and invasion in renal cell carcinoma

Fig. 5

LncRNA RP11-436H11.5 works as a miR-335-5p decoy to regulate BCL-W expression in RCC cells. a. Sequence alignment of lncRNA RP11-436H11.5 3′-UTR with wild-type (WT) versus mutant potential miR-335-5p targeting sites. b. Cotransfection of wild-type or mutant seed regions of lncRNA RP11-436H11.5 3′-UTR constructed with oemiR-335-5p in A498 and 786-O cells. Luciferase reporter assay was applied to detect the luciferase activities. c. A RNA-pull down assay was performed by biotinylated antisense oligo specific to lncRNA RP11-436H11.5 and BCL-W after transfecting with oelncRNA RP11-436H11.5 or shlncRNA RP11-436H11.5 in A498 and 786-O cells. d. Cotransfection of wild-type or mutant seed regions of BCL-W 3′-UTR constructed with oemiR-335-5p and oelncRNA RP11-436H11.5 in A498 cells. Luciferase reporter assay was applied to detect luciferase activities. e. Cotransfection of wild-type or mutant seed regions of BCL-W 3′-UTR constructed with miR-335-5p inhibitor and shlncRNA RP11-436H11.5 in 786-O cells. Luciferase reporter assay was applied to detect the luciferase activities. b, c, d and e. Each sample was run in triplicate and in multiple experiments for mean ± SEM. *P < 0.05, **P < 0.01 compared to controls

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